Clostridium perfringens Spores in Urology Hospital

Background: Clostridium spp. spores are resistant to many factors, including alcohol-based disinfectants. The presence of clostridial in a hospital environment may lead infection outbreaks among patients and health care workers. Objective: This study is aimed detect the aurology using C diff Banana Broth™ assess antibiotic sensitivity toxinotypes isolates. Methods: After diagnosing COVID-19 medical staff closing an 86-bed urology 2020 for H2O2 fogging, 58 swabs from were inoculated Broth™, incubated at 37°C 14 days, checked daily, positive broths sub-cultured anaerobically 48 h 37°C. identification, multiplex PCR (mPCR) was performed perfringens, C. difficile toxin genes, minimum inhibitory concentration (MIC) determination. Results: In this study, 16 out (~ 28%) strains cultured: 11 - 2 baratii, 1 each paraputrificum, difficile, clostridioforme. perfringens cpa, 7 cpb2, – cpiA, cpb genes. All isolates sensitive metronidazole, vancomycin, moxifloxacin, penicillin/tazobactam, rifampicin. Two erythromycin clindamycin. Conclusions: Regardless antibiotic-resistant, toxigenic (69%) obtained cultured indicating need develop necessary sanitary epidemiological procedures hospital.